Organic Fertilizer Brown Powder N-Acetyl D-Glucosamine Agriculture Benefits On Rice Growing

Organic Fertilizer Brown Powder N-Acetyl D-Glucosamine Agriculture Benefits On Rice Growing​

General Information of N-Acetyl-D-Glucosamine

Natural amino monosaccharides (amino oligosaccharin monomers), which only exist in polysaccharide molecules in organisms or in a free state, industrial products are N-acetylglucosamine (chitin monosaccharide) and glucosamine hydrochloride

Glucosamine is the basic substance for the synthesis of many important polysaccharides in organisms. It not only plays a prominent role in the field of medicine, but also as a new synergist, it also has outstanding performance in promoting root, growth and stress resistance of plants.

Application of N-Acetyl D-Glucosamine

Plants often face living things as they grow stress (virus, insect, fungus, etc.) and abiotic stress (cold damage, droughtHarm, salinity, mineral deficiency, heavy metals, etc.) pressure, which temperature Is one of the important conditions for plant growth and development (Lukatkin et al.,2012). As a subtropical thermotropic plant, rice is sensitive to low temperature environment Low temperature can cause stunted growth and even death of rice seedlings, Yan Weight affects rice yield (Hussain et al., 2016). To improve the plant Cold resistance, abscisic acid, polybulozole, chlorcholine and other plant growth regulation anticold agents and betaine, polyamine and other compounds have been widely used as anticold agents Application (Hussain et al., 2018).

Chemical anticold agent is more effective Good, but easy to produce chemical residue and harm plant growth, resulting in environmental pollution Dye. So development is less threatening to plants, green, safe and good. The economic benefit of plant immune and resistant agents is particularly important. Now, the carapace As a nitrogenous polysaccharide, tin and its derivatives are safe, green and so on Potential has been widely used in the field of immunoinducement, N- acetyl amino grape N-acetyl-D-glucosamine (NAG) is a molecule of glucose An amino hexose in which the hydroxyl group on C2 is replaced by an acetyl group Chitin into the structural unit of the substance, but its immune-induced resistance collar in plants. The application research of domain is relatively rare.

Test Material and Method:

Plant cultivation and treatment Select large and full grains, uniform grains the uniform rice seeds are placed in clear water and fully soaked, and the rice seeds that sink to the bottom seeds with better quality were rinsed with water and soaked in 75% ethanol solution.

Disinfect with water for 15 minutes, rinse with water several times until no ethanol remains. Will rice seeds were soaked in deionized water at 30 °C for 48 h, and then germinate at 30°C for 24 hours in the dark. After it is exposed, carefully choose the one that is exposed.

The cultivated rice seeds were transferred to 96-well plates for culture, and then transplanted to hydroponics in an incubator. Culture conditions: temperature 25 ℃, The relative humidity is 75%, and the photoperiod is 16 h/8 h (day/night). to promote water Rice seeds obtain nutrition in time, combined with the actual situation in the process of seedling cultivation growth state, after about 7 days in hydroponics (deionized water), replace with nutrient solution for culture (The nutrient solution is formulated according to the nutrient solution formula developed by the International Rice Research Institute.Set), change the medium every 2 days;

Add when the seedlings grow to the stage of 2 leaves and 1 heart (about 14 days in total)

NAG carries out root system culture, the specific method is as follows: (1) The effect of cold resistance is strong for degree screening, NAG treatment levels were 0 (control), 50, 100 and 150mg/L. Start seedlings under light conditions in the photoperiod root culture of sugar, after 48 hours of sugar treatment, low temperature stress treatment (at a temperature of 5°C) for 24 h. (2) When studying the mechanism of cold resistance, based on screening the optimal NAG treatment concentration obtained, the seedlings were divided into normal temperature control group(No NAG root treatment seedlings, cultivated at room temperature), room temperature treatment group (Apply the optimal concentration of NAG to root treatment seedlings, culture at room temperature), low temperature control group (no NAG root treatment seedlings, 5 ℃ low temperature stress culture 24 h) and the low temperature treatment group (root treatment with optimal NAG concentration seedlings were cultured under low temperature stress at 5 °C for 24 h). Then in the light treatment bar samples were taken under conditions, frozen in liquid nitrogen and stored in a -80 °C refrigerator for determination subsequent related indicators.

Determination of seedling growth index 40~50 fresh rice seedlings were randomly selected, and the roots of the seedlings were gently wiped with filter paper to absorb the roots.

The surface moisture of the part was determined to determine the fresh weight of a single seedling. The distance from the base of the seedling root to the top of the topmost unfolded leaf sheath and the distance to the root tip were determined respectively, that is, the seedling plant height and root length.

Results & Analysis

Effect of NAG on cold tolerance of rice seedlings

Effects of Different Concentrations of NAG on Growth of Rice Seedlings under Low Temperature Stress After root culture with different concentrations of NAG, the seedlings were cold-affected seedlings. The overall growth (Figure 1-A) showed that after low temperature and cold damage, the seedlings of the 0 mg/L control group wilted and did not stand upright, the leaf morphology changed, the leaf margin lost water, and the leaf tip was curled. However, the seedlings cultured with 50 and 100 mg/L NAG root systems had a more erect phenotype, brighter green leaf color, and better overall growth status than the control group. The results showed that exogenous application of NAG could alleviate the damage of low temperature to rice and protect rice seedlings

It can be seen from Figs. 1-B and Fig. 1-C that compared with the 0 mg/L control group, the fresh weight of seedlings increased by 11.94%, 14.14% and 9.93%, respectively, after 50, 100 and 150 mg/L NAG treatment. The plant height increased by 3.81%, 7.14% and 6.38%, respectively, and all of them were significantly different from those in the control group (P<0.05, the same below). From Figure 1-D, it can be seen that there was no significant difference between the root length of seedlings after 50 mg/L NAG root culture and the control group P >0.05, the same below), while the root length of seedlings after 100 mg/L NAG root culture increased significantly by 7.22% and 5.87% compared with the control group, respectively. It can be seen that NAG root treatment has a significant effect on promoting the growth of seedlings under low temperature conditions, and can protect the normal growth of seedlings, among which the 100 mg/L NAG treatment group has the best effect of restoring growth, and the fresh weight of a single plant (0.118±0.009 g), plant height (21.21±0.72 cm) and root length (5.76±0.43 cm) are the maximums.

Effects of different concentrations of NAG on chlorophyll and soluble sugar content of rice seedlings under low temperature stress Figures 2-A and 2-B were shown respectively. the changes of chlorophyll content in seedlings cultured with different concentrations of NAG root system after 24 h of low temperature stress and recovery of growth at normal temperature after low temperature stress. It can be seen from Fig. 2-A that compared with the 0 mg/L control group, the contents of chlorophyll a, chlorophyll b and total chlorophyll in cold seedlings treated with 50, 100 and 150 mg/L NAG showed an increasing trend, and there were significant differences between different treatment groups of the same index, among which 100 mg/L NAG. The chlorophyll a content (4.253±0.016 mg/g), chlorophyll b content (1.707±0.008 mg/g) and total chlorophyll content (5.959±0.020 mg/g) increased the most than that of the control group, with significant increases of 36.65%, 37.13% and 36.79%, respectively. It was shown that NAG root treatment could promote cold damage.

The synthesis of chlorophyll in seedlings protects seedlings from normal photosynthesis to resist cold damage. It can be seen from Figure 2-B that 100 mg/L NAG treatment can also significantly promote the accumulation of chlorophyll in the recovery of cold seedlings during the recovery process, and ensure subsequent normal growth, and the contents of chlorophyll a, chlorophyll b and total chlorophyll in the seedlings in the control group were significantly increased by 14.76%, 20.17% and 18.94%, respectively.

It can be seen from Figure 2-C that root treatment of 50, 100 and 150 mg/L NAG promoted the accumulation of soluble sugars of osmomodulating substances in seedlings at low temperature, which increased by 23.26%, 63.09% and 40.46%, respectively, compared with the control group

Among them, the soluble sugar content (41.031±0.992 μg/g) in the 100 mg/L NAG treatment group had the most obvious growth trend, and the differences between the treatment groups reached a significant level. The results showed that NAG could effectively regulate the infiltration potential of cold seedlings, thereby protecting seedlings against cold damage. The growth potential, growth index, and chlorophyll content of seedlings were comprehensively compared

and soluble sugar accumulation, it was found that during the process of low temperature stress, exogenous application of a certain concentration of NAG could protect the normal growth of rice seedlings in low temperature environment and improve the cold resistance effect of rice. From the cold resistance effect and economic benefits of different concentrations of NAG, 100 mg/L in this study was

Optimal concentration for application.

Conclusion:

Under low temperature stress, exogenous application of NAG by root treatment could protect rice seedlings, restore the accumulation of chlorophyll during growth after low temperature stress, and promote their normal growth in low temperature environment. The possible regulatory mechanism is as follows: first, NAG regulates the osmotic potential of rice seedlings, promotes the accumulation of osmoregulatory substances and the conversion and utilization of nitrate nitrogen; Secondly, the exogenous application of NAG effectively reduced the MDA content in cold seedlings, increased the activity of SOD and POD antioxidant enzymes, and protected the antioxidant system of seedlings. In addition, NAG significantly promoted the gene table of OsNCED, OsSDR and AAO in the abscisic acid metabolism pathway of cold seedlings
In turn, it promotes the accumulation of endogenous abscisic acid in seedlings, and protects seedlings against cold damage through abscisic acid metabolism.

Packaging and storage of N-Acetyl D-Glucosamine

Packaging and storage Available in 1Kg, 5Kg, 10Kg, 20Kg drum;

Storage: Dry, cool, direct sun light proof, moisture proof warehouse;

Shelf life: 24 months.